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bcl 2 associated x protein bax  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc bcl 2 associated x protein bax
    Bcl 2 Associated X Protein Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcl+2+associated+x+protein/pmc12657607-90-26-31
    Average 86 stars, based on 1 article reviews
    bcl 2 associated x protein bax - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    Aqueous Normal-phase Chromatography:

    Article Title: ABCC9 knockdown attenuates isoproterenol-induced myocardial hypertrophy by inhibiting the PI3K/AKT signaling pathway
    Article Snippet: An anti-ABCC9 antibody (cat. no. 40229) was purchased from Signalway Antibody LLC. .. Antibodies against B-cell lymphoma 2 protein (Bcl-2; cat. no. 15071T), Bcl-2-associated X protein (Bax; cat. no. 2772T), caspase-3 (cat. no. 9662S), cleaved caspase-3 (cat. no. 9661S), phosphorylated (p)-PI3K (cat. no. 17366T), PI3K (cat. no. 4292S), p-AKT (cat. no. 9271T), AKT (cat. no. 4691T) and β-actin (cat. no. 3700S) were purchased from Cell Signaling Technology, Inc. Atrial natriuretic peptide (ANP; cat. no. ab189921) and brain natriuretic peptide (BNP; cat. no. ab236101) were purchased from Abcam. .. GAPDH (cat. no. sc-32233) and secondary antibodies were purchased from Santa Cruz Biotechnology, Inc., including mouse anti-rabbit IgG-HRP (cat. no. sc-2357) and mouse IgGκ light chain binding protein conjugated to HRP (cat. no. sc-516102).

    Immunohistochemical staining:

    Article Title: Prohibitin 2 ameliorates cisplatin-induced acute kidney injury by modulating mitochondrial homeostasis
    Article Snippet: .. Antibodies specific for Bcl-2-associated X protein (Bax; 2772), microtubule-associated protein light chain 3 (LC3; 12741T), cleaved caspase-3 (9661 for immunohistochemical staining), and β-tubulin (2128T) were procured from Cell Signaling Technology. .. A mouse monoclonal antibody targeting PHB2 (sc-133094) was sourced from Santa Cruz Biotechnology.

    Staining:

    Article Title: Prohibitin 2 ameliorates cisplatin-induced acute kidney injury by modulating mitochondrial homeostasis
    Article Snippet: .. Antibodies specific for Bcl-2-associated X protein (Bax; 2772), microtubule-associated protein light chain 3 (LC3; 12741T), cleaved caspase-3 (9661 for immunohistochemical staining), and β-tubulin (2128T) were procured from Cell Signaling Technology. .. A mouse monoclonal antibody targeting PHB2 (sc-133094) was sourced from Santa Cruz Biotechnology.

    Blocking Assay:

    Article Title: Oral Administration of Astrocyte-Targeted Natural Antioxidants Suppress NOX4-Driven Neuroinflammation and Restore Hippocampal Neurogenesis in MPTP-Induced Parkinson’s Disease Mouse Model
    Article Snippet: Protein concentrations were measured, and equal amounts were subjected to SDS-PAGE and transferred onto PVDF membranes (Cytiva, Marlborough, MA, USA). .. After blocking with TBST containing 5% BSA, membranes were incubated overnight (4 °C) with primary antibodies: 4-Hydroxynonenal (4-HNE; 1:1000, Bioss, Beijing, China), Bcl-2-Associated X Protein (BAX; 1:1000, Cell signaling Technology), B-Cell Lymphoma 2 (Bcl-2; 1:1000, Cell signaling Technology), cleaved caspase-3 (CC3; 1:500, Cell Signaling Technology), DCX (1:1000, Abcam), GFAP (1:1000, Merck Millipore, Burlington, MA, USA), Ionized calcium binding adaptor molecule 1 (Iba-1; 1:200, Wako, Osaka, Japan), MPO (1:500, R&D systems), OPN (1:100, R&D Systems), NeuN (1:500, Abcam), NOX4 (1:100, Novus, Centennial, CO, USA), PSD95 (1:1000, Invitrogen, Waltham, MA, USA), Synaptophysin (1:20,000, Novus), TIM23 (1:1000, Proteintech, Chicago, IL, USA), Tom20 (1:100, Santa Cruz Biotechnology), Total OXPHOS (1:250, Abcam) and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH; 1:10,000, Cell Signaling). .. After washing, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Vector Laboratories, Cell Signaling) for 2 h, and immunoreactive bands were visualized using enhanced chemiluminescence reagents (BS ECL Plus Kit; Biosesang, Yongin-si, Gyeonggi-do, Republic of Korea).

    Incubation:

    Article Title: Oral Administration of Astrocyte-Targeted Natural Antioxidants Suppress NOX4-Driven Neuroinflammation and Restore Hippocampal Neurogenesis in MPTP-Induced Parkinson’s Disease Mouse Model
    Article Snippet: Protein concentrations were measured, and equal amounts were subjected to SDS-PAGE and transferred onto PVDF membranes (Cytiva, Marlborough, MA, USA). .. After blocking with TBST containing 5% BSA, membranes were incubated overnight (4 °C) with primary antibodies: 4-Hydroxynonenal (4-HNE; 1:1000, Bioss, Beijing, China), Bcl-2-Associated X Protein (BAX; 1:1000, Cell signaling Technology), B-Cell Lymphoma 2 (Bcl-2; 1:1000, Cell signaling Technology), cleaved caspase-3 (CC3; 1:500, Cell Signaling Technology), DCX (1:1000, Abcam), GFAP (1:1000, Merck Millipore, Burlington, MA, USA), Ionized calcium binding adaptor molecule 1 (Iba-1; 1:200, Wako, Osaka, Japan), MPO (1:500, R&D systems), OPN (1:100, R&D Systems), NeuN (1:500, Abcam), NOX4 (1:100, Novus, Centennial, CO, USA), PSD95 (1:1000, Invitrogen, Waltham, MA, USA), Synaptophysin (1:20,000, Novus), TIM23 (1:1000, Proteintech, Chicago, IL, USA), Tom20 (1:100, Santa Cruz Biotechnology), Total OXPHOS (1:250, Abcam) and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH; 1:10,000, Cell Signaling). .. After washing, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Vector Laboratories, Cell Signaling) for 2 h, and immunoreactive bands were visualized using enhanced chemiluminescence reagents (BS ECL Plus Kit; Biosesang, Yongin-si, Gyeonggi-do, Republic of Korea).

    Binding Assay:

    Article Title: Oral Administration of Astrocyte-Targeted Natural Antioxidants Suppress NOX4-Driven Neuroinflammation and Restore Hippocampal Neurogenesis in MPTP-Induced Parkinson’s Disease Mouse Model
    Article Snippet: Protein concentrations were measured, and equal amounts were subjected to SDS-PAGE and transferred onto PVDF membranes (Cytiva, Marlborough, MA, USA). .. After blocking with TBST containing 5% BSA, membranes were incubated overnight (4 °C) with primary antibodies: 4-Hydroxynonenal (4-HNE; 1:1000, Bioss, Beijing, China), Bcl-2-Associated X Protein (BAX; 1:1000, Cell signaling Technology), B-Cell Lymphoma 2 (Bcl-2; 1:1000, Cell signaling Technology), cleaved caspase-3 (CC3; 1:500, Cell Signaling Technology), DCX (1:1000, Abcam), GFAP (1:1000, Merck Millipore, Burlington, MA, USA), Ionized calcium binding adaptor molecule 1 (Iba-1; 1:200, Wako, Osaka, Japan), MPO (1:500, R&D systems), OPN (1:100, R&D Systems), NeuN (1:500, Abcam), NOX4 (1:100, Novus, Centennial, CO, USA), PSD95 (1:1000, Invitrogen, Waltham, MA, USA), Synaptophysin (1:20,000, Novus), TIM23 (1:1000, Proteintech, Chicago, IL, USA), Tom20 (1:100, Santa Cruz Biotechnology), Total OXPHOS (1:250, Abcam) and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH; 1:10,000, Cell Signaling). .. After washing, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Vector Laboratories, Cell Signaling) for 2 h, and immunoreactive bands were visualized using enhanced chemiluminescence reagents (BS ECL Plus Kit; Biosesang, Yongin-si, Gyeonggi-do, Republic of Korea).



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    Image Search Results


    The effects of RSV on the expression of Bcl-2, cleaved caspase-3, Bax, Nrf2, HO-1, TLR4, and NF-κB after renal I/R. (A) Representative Western blotting and (B–H) Quantification of Western blotting data. Data are expressed as mean ± SD. * p < 0.05 versus DS group; # p < 0.05 versus DI/R group; ■ p < 0.05 versus DI/R + RSV group.

    Journal: Renal Failure

    Article Title: Resveratrol inhibits renal ischemia and reperfusion injury in diabetes via reducing oxidative stress, inflammation, and apoptosis

    doi: 10.1080/0886022X.2026.2652103

    Figure Lengend Snippet: The effects of RSV on the expression of Bcl-2, cleaved caspase-3, Bax, Nrf2, HO-1, TLR4, and NF-κB after renal I/R. (A) Representative Western blotting and (B–H) Quantification of Western blotting data. Data are expressed as mean ± SD. * p < 0.05 versus DS group; # p < 0.05 versus DI/R group; ■ p < 0.05 versus DI/R + RSV group.

    Article Snippet: The blots were blocked with 5% skimmed milk and incubated with primary antibodies against Nrf2 (cat. no. 16396-1-AP; 1:2,000), NF-κB p65 (cat. no. 80979-1-RR; 1:1,000), HO-1 (cat. no. ab68477; 1:1,000), lamin B (cat. no. 66095-1-Ig; 1:20,000), β-actin (cat. no. 60004-1-Ig; 1:5,000; all Proteintech Group, Inc.), TLR4 (cat. no. AF7017; 1:1,000; Affinity Biosciences), B-cell lymphoma-2 (Bcl-2; cat. no. GB154830 ; 1:1,000; Servicebio), Bcl-2-associated X protein (Bax; cat. no. BS-0127R; 1:1,000; BIOSS), and cleaved caspase-3 (cat. no. 9661 T; 1:1,000; Cell Signaling Technology), respectively.

    Techniques: Expressing, Western Blot

    The effects of RSV on the expression of Bcl-2, cleaved caspase-3, Bax, Nrf2, HO-1, TLR4, and NF-κB in HK-2 cells after H/R. (A) Representative Western blotting and (B–H) Quantification of Western blotting data. Data are expressed as mean ± SD. * p < 0.05 versus Control group; # p < 0.05 versus H/R group; ■ p < 0.05 versus H/R + RSV group.

    Journal: Renal Failure

    Article Title: Resveratrol inhibits renal ischemia and reperfusion injury in diabetes via reducing oxidative stress, inflammation, and apoptosis

    doi: 10.1080/0886022X.2026.2652103

    Figure Lengend Snippet: The effects of RSV on the expression of Bcl-2, cleaved caspase-3, Bax, Nrf2, HO-1, TLR4, and NF-κB in HK-2 cells after H/R. (A) Representative Western blotting and (B–H) Quantification of Western blotting data. Data are expressed as mean ± SD. * p < 0.05 versus Control group; # p < 0.05 versus H/R group; ■ p < 0.05 versus H/R + RSV group.

    Article Snippet: The blots were blocked with 5% skimmed milk and incubated with primary antibodies against Nrf2 (cat. no. 16396-1-AP; 1:2,000), NF-κB p65 (cat. no. 80979-1-RR; 1:1,000), HO-1 (cat. no. ab68477; 1:1,000), lamin B (cat. no. 66095-1-Ig; 1:20,000), β-actin (cat. no. 60004-1-Ig; 1:5,000; all Proteintech Group, Inc.), TLR4 (cat. no. AF7017; 1:1,000; Affinity Biosciences), B-cell lymphoma-2 (Bcl-2; cat. no. GB154830 ; 1:1,000; Servicebio), Bcl-2-associated X protein (Bax; cat. no. BS-0127R; 1:1,000; BIOSS), and cleaved caspase-3 (cat. no. 9661 T; 1:1,000; Cell Signaling Technology), respectively.

    Techniques: Expressing, Western Blot, Control